The OPTOStokes CY3/TRITC Fluorescence Filter Set is the industry-standard optical solution for visualizing orange-red fluorophores in wide-field fluorescence microscopy. Specifically calibrated for CY3, TRITC (Tetramethylrhodamine), and TAMRA, this set ensures maximum excitation efficiency at 530nm while capturing the peak emission at 605nm. Engineered with hard-coated technology, it delivers exceptional signal brightness and contrast for demanding imaging applications.
Technical Specifications
Designed for durability and spectral precision, our fluorescence microscopy filters feature steep transition edges and deep blocking to eliminate crosstalk from adjacent channels (such as DAPI or FITC).
| Component | Model | Center Wavelength (CWL) | FWHM | Transmission | Blocking (OD) |
|---|---|---|---|---|---|
| Excitation | BP530-40nm | 530 ± 2nm | 40 ± 2nm | Tave > 90% (515-545nm) | ODave ≥ 6 (350-500nm) ODave ≥ 6 (560-800nm) OD > 3 (800-1040nm) |
| Emission | BP605-55nm | 605 ± 2nm | 55 ± 2nm | Tave > 90% (580-630nm) | ODave ≥ 6 (350-570nm) ODave ≥ 6 (645-830nm) OD > 3 (830-1050nm) |
| Dichroic | DM565 | Cut-on ~565nm | N/A | T > 95% (575-700nm) | T < 2% (430-555nm) @ 45° AOI |
Primary Applications
This 530nm filter based set is optimized for high-contrast imaging in:
- Fluorescence Microscopy: Routine observation of cytoskeleton structures labeled with TRITC-Phalloidin.
- FISH Detection: Critical for analyzing specific DNA sequences using orange/red labeled probes in FISH detection assays.
- Pathology: Identification of pathogens and cellular components using immunofluorescence.
- Single-Molecule Imaging: Applications requiring high photon collection efficiency for CY3 derivatives.
Customization & Microscope Compatibility
OPTOStokes offers extensive custom optical filters services to ensure our optics fit your specific hardware.
- Filter Cubes: We can provide filters mounted in anodized aluminum rings to fit standard filter cubes from major microscope manufacturers (Nikon, Olympus, Zeiss, Leica).
- Spectral Tuning: Custom CWL (400nm-850nm) and FWHM (10nm-60nm) to match novel fluorophores.
- Background Suppression: For applications requiring extreme darkness, we offer high OD filter options with ODabs ≥ 5 and ODave ≥ 6 across the detector's sensitivity range.
Why Choose OPTOStokes?
Contrast is Key: In fluorescence microscopy, the background is just as important as the signal. Our filter sets utilize superior blocking technologies to ensure that only the specific fluorescence signal reaches the camera or eyepiece.
Rapid Fulfillment: Whether you need a standard set for a university lab or volume OEM production, our reliable stock and manufacturing capacity ensure you get the optics you need, when you need them.
Upgrade your imaging system today. Contact our optical engineers at sales@optofilters.com for technical diagrams or to request a quote.
