The OPTOStokes CY5 / Alexa Fluor 647 Filter Set is a high-performance optical solution designed for the far-red spectral region. Optimized for CY5, Alexa Fluor 647, and Draq5 dyes, this set minimizes crosstalk and background noise, which is critical for far-red imaging where detector sensitivity can be a limiting factor. By combining steep spectral edges with deep blocking, we ensure maximum signal capture for wide-field fluorescence microscopy and fluorescence detection systems.
Technical Specifications
Engineered with dense, hard optical coating technology, these filters offer exceptional environmental durability and spectral precision.
| Component | Model | Center Wavelength (CWL) | FWHM | Transmission | Blocking (OD) |
|---|---|---|---|---|---|
| Excitation | BP624-40nm | 624 ± 2nm | 40 ± 2nm | Tave > 90% (610-640nm) | ODave ≥ 6 (350-595nm) ODave ≥ 6 (655-860nm) OD > 3 (860-1050nm) |
| Emission | BP694-40nm | 694 ± 2nm | 40 ± 2nm | Tave > 90% (680-708nm) | ODave ≥ 6 (350-664nm) ODave ≥ 6 (730-800nm) OD > 3 (800-1050nm) |
| Dichroic | DM655 | Cut-on ~655nm | N/A | T > 95% (670-700nm) | T < 2% (430-645nm) @ 45° AOI |
Primary Applications
This red filter set is essential for applications requiring distinct channel separation in multiplex assays:
- Immunofluorescence: High-contrast imaging of cytoskeletal and nuclear structures labeled with Far-Red dyes.
- Super-Resolution Microscopy: Suitable for STORM and other localization microscopy techniques using Alexa Fluor 647.
- Flow Cytometry: Precise bandpass control for the Red Laser (633/640nm) channel.
- DNA Sequencing: Reliable separation of long-wavelength fluorophores.
Customization & Manufacturing
OPTOStokes provides extensive custom optical filters services to adapt this set to your specific instrument or filter cube requirements.
- Mechanical Integration: Available in standard sizes (6x6mm, Ø12.5mm, Ø25mm) and custom shapes. We offer anodized aluminum rings for seamless mounting into Nikon, Olympus, Zeiss, and Leica filter sets.
- Spectral Tuning: We can adjust CWL (400-850nm) and FWHM (10-60nm) to match specific light sources or detectors.
- Deep Blocking: For high-sensitivity sensors, we ensure high OD filter performance with ODabs ≥ 5 and ODave ≥ 6 suppression across the UV-NIR range (350-800nm).
Why Choose OPTOStokes?
Precision & Availability: We combine world-class coating precision with an extensive in-stock selection. Whether you need a single prototype for R&D or volume production for OEM instruments, we guarantee reliable lead times and consistent quality.
Need a tailored optical solution? Contact our engineering team at sales@optofilters.com to request a quote or discuss your specific microscopy requirements.
