The OPTOStokes ROX / mCherry / Alexa Fluor 594 Filter Set is engineered for peak performance in the orange-to-red spectrum, targeting popular fluorophores like mCherry, ROX (Reference), and Alexa Fluor 594. This set is optimized to maximize signal collection from dyes with broad emission curves, such as mCherry, utilizing an exceptionally wide 60nm emission bandpass. Simultaneously, the deep optical density (OD) blocking minimizes unwanted signal and noise, delivering the clean background required for high-precision fluorescence detection and quantitative PCR.
Technical Specifications for High-Performance Imaging
Each component features robust, hard-coated optical coatings, guaranteeing long-term stability and consistent spectral performance across the UV to NIR ranges.
| Component | Model | Center Wavelength (CWL) | FWHM | Transmission | Blocking (OD) |
|---|---|---|---|---|---|
| Excitation | BP560-40nm | 560 ± 3nm | 40 ± 3nm | Tave > 90% (545-575nm) | ODave ≥ 6 (200-530nm) ODave ≥ 6 (590-800nm) OD > 3 (800-1050nm) |
| Emission | BP635-60nm | 635 ± 2nm | 60 ± 2nm | Tave > 90% (610-660nm) | ODave ≥ 6 (200-595nm) ODave ≥ 6 (680-800nm) OD > 3 (800-1050nm) |
| Dichroic Mirror | DM600 | Cut-off ~600nm | N/A | T > 95% (615-700nm) | T < 2% (430-595nm) @ 45° AOI |
Target Applications
The unique spectral configuration of these filter sets makes them indispensable for:
- qPCR and Real-Time PCR: Essential for utilizing ROX as a passive reference dye for accurate data normalization in PCR fluorescence systems.
- Live Cell and Confocal Microscopy: Ideal for high-contrast imaging of mCherry and other red fluorescent proteins.
- Multiplexed Assays: Deep high OD filter blocking ensures minimal signal bleed-through when combining with other channels (e.g., GFP or FITC).
Customization and Reliable Supply Chain
OPTOStokes understands that system integration requires flexibility. We offer both an extensive inventory of in-stock components and robust support for high-level customization.
- Custom Dimensions: Standard formats include 1.0mm or 1.1mm thickness, and sizes from 6x6mm up to Ø25mm, ready to be fitted with aluminum mounting rings if required.
- Spectral Adjustments: Our engineers can provide custom bandpass modifications to CWL (400-850nm) and FWHM (10-60nm) to perfectly match novel dyes or unique laser specifications.
Don't compromise on signal quality or lead time. Leverage OPTOStokes' world-class quality assurance and reliable supply for your project. Contact our technical sales team at sales@optofilters.com for immediate inventory checks or to begin designing your custom optical solution today.
