The OPTOStokes FAM Fluorescence Filter Set is the industry standard for detecting FAM, FITC, and SYBR Green dyes in molecular diagnostics. Engineered for high-throughput PCR fluorescence systems, this set balances maximum transmission with ultra-deep blocking. By isolating the 470nm blue excitation line and the 520nm green emission signal, we ensure exceptional signal-to-noise ratios essential for low-copy-number detection.
Technical Specifications
Our filters utilize hard-coated technology to deliver environmental durability and consistent spectral performance. The set includes a precise blue bandpass filter for excitation and a matched green emission filter.
| Component | Model | Center Wavelength (CWL) | FWHM | Transmission | Blocking (OD) |
|---|---|---|---|---|---|
| Excitation | BP470-30nm | 470 ± 2nm | 30 ± 2nm | Tave > 90% (460-480nm) | ODave ≥ 6 (350-440nm) ODave ≥ 6 (497.5-800nm) OD > 3 (800-1050nm) |
| Emission | BP520-20nm | 520 ± 2nm | 20 ± 2nm | Tave > 90% (515-525nm) | ODave ≥ 6 (350-500nm) ODave ≥ 6 (540-800nm) OD > 3 (800-1050nm) |
| Dichroic | DM505 | Cut-on ~505nm | N/A | T > 95% (515-745nm) | T < 2% (380-495nm) @ 45° AOI |
Critical Applications
This optical configuration is optimized for instruments requiring high optical clarity and background suppression:
- Real-Time PCR (qPCR): Standard channel for FAM and SYBR Green assays.
- Fluorescence Microscopy: Ideal for GFP (Green Fluorescent Protein) and FITC imaging.
- DNA Sequencing: Precision 520nm filter separation for genetic analysis.
- Point-of-Care Devices: Compact, reliable optics for portable diagnostics.
Customization & Engineering Support
OPTOStokes provides flexible custom optical filters services to adapt to your specific instrument design.
- Spectral Tuning: We can adjust CWL from 400nm to 850nm and FWHM from 10nm to 60nm.
- Deep Blocking: Our high OD filter capabilities ensure ODabs ≥ 5 and ODave ≥ 6 across the UV-NIR range (350-800nm).
- Mechanical Integration: Standard thickness (1.0mm/1.1mm) and sizes (6x6mm, Ø6mm, Ø12mm, Ø25mm). Anodized aluminum rings are available for easy mounting.
Why Partner with OPTOStokes?
Eliminate Crosstalk: In multi-channel PCR, signal bleed-through ruins data. Our filters feature steep edges and OD6 blocking to guarantee channel isolation.
From Prototype to Production: Whether you need a single verification set or volume production of filter sets, our inventory and agile manufacturing ensure reliable lead times.
Upgrade your optical system today. Contact our technical team at sales@optofilters.com to request samples or discuss your custom optical design requirements.
