The OPTOStokes ROX Fluorescence Filter Set is meticulously engineered for the detection of ROX (Carboxy-X-Rhodamine) and Texas Red fluorophores. widely utilized as a passive reference dye in quantitative PCR (qPCR) to normalize non-PCR-related fluctuations. This optical set delivers exceptional spectral isolation, ensuring stable baselines and accurate data quantification by suppressing crosstalk from adjacent channels (such as HEX or FAM).
Technical Specifications
Manufactured with dense, hard-coated optical glass, our filters offer superior environmental stability. The set comprises a narrowband excitation filter, a matched emission filter, and a dichroic mirror optimized for 45° incidence.
| Component | Model | Center Wavelength (CWL) | FWHM | Transmission | Blocking (OD) |
|---|---|---|---|---|---|
| Excitation | BP570-20nm | 570 ± 2nm | 20 ± 2nm | Tave > 90% (565-575nm) | ODave ≥ 6 (350-550nm) ODave ≥ 6 (591-800nm) ODave > 3 (800-1050nm) |
| Emission | BP612-20nm | 612 ± 2nm | 20 ± 2nm | Tave > 90% (607-617nm) | ODave ≥ 6 (350-591.5nm) ODave ≥ 6 (632-800nm) OD > 3 (800-1050nm) |
| Dichroic | DM590 | Cut-on ~590nm | N/A | Tave > 95% (625-850nm) | Tave < 2% (390-580nm) @ 45° AOI |
Primary Applications
This red filter set is a critical component for high-precision bio-instrumentation:
- Real-Time PCR (qPCR): Standard channel for ROX reference dye normalization.
- Fluorescence Microscopy: High-contrast imaging of Texas Red and similar orange-red fluorophores.
- DNA Sequencing: Precise separation of dye-terminator signals.
- Pathogen Detection: Reliable PCR fluorescence optics for multiplex diagnostic platforms.
Customization & Manufacturing Capabilities
OPTOStokes offers comprehensive custom optical filters services to fit your instrument's unique optical path and mechanical housing.
- Spectral Range: CWL selectable from 400nm to 850nm; FWHM from 10nm to 60nm.
- Deep Blocking: We ensure ODabs ≥ 5 and ODave ≥ 6 across the UV-NIR spectrum (350-800nm) for critical high OD filter requirements.
- Dimensions & Mounting: Standard sizes include 6x6mm, Ø6mm, Ø12mm, and Ø25mm. We provide anodized aluminum rings and custom shapes upon request.
Why Choose OPTOStokes?
Signal Integrity: In multiplex PCR, separating the ROX signal from background noise is paramount. Our bandpass filter technology utilizes steep edges to minimize spectral overlap.
Reliable Supply Chain: From rapid prototyping to volume production, we maintain extensive stock and robust manufacturing processes to guarantee on-time delivery.
Optimizing your optical detection system? Contact our engineering team at sales@optofilters.com for technical support or to request a quote for your custom filter sets.
