The OPTOStokes HEX Fluorescence Filter Set is specifically designed for high-performance detection of the HEX fluorophore, a key component in multiplex quantitative Polymerase Chain Reaction (qPCR) and various bio-imaging applications. This set ensures optimal excitation at 530nm and emission capture at 565nm, critical for accurate discrimination in multi-channel systems. Engineered for high signal-to-noise ratio, these filters deliver the precision required by R&D engineers.
Technical Specifications
Our filters utilize hard high precision coating on the substrate, guaranteeing spectral stability and durability. The complete set includes matched excitation and emission bandpass filters, along with a high-efficiency dichroic mirror.
| Component | Model | Center Wavelength (CWL) | FWHM | Transmission | Blocking (OD) |
|---|---|---|---|---|---|
| Excitation | BP530-20nm | 530 ± 2nm | 20 ± 2nm | Tave > 90% (525-535nm) | ODave ≥ 6 (350-510nm) ODave ≥ 6 (550-800nm) OD > 3 (800-1050nm) |
| Emission | BP565-22nm | 565 ± 2nm | 22 ± 2nm | Tave > 90% (560-570nm) | ODave ≥ 6 (200-547nm) ODave ≥ 6 (585-800nm) OD > 3 (800-1050nm) |
| Dichroic | DM550 | Cut-on ~550nm | N/A | Tave > 95% (560-700nm) | Tave < 2% (430-538nm) @ 45° AOI |
Key Applications
This high-fidelity green light filter set is essential for maximizing signal clarity in complex fluorescence assays, including:
- Multiplex qPCR: Isolating the HEX signal from adjacent fluorophores like FAM and ROX/Cy3.
- Fluorescence Microscopy: Imaging of dyes such as VIC, JOE, and Cy2.
- Flow Cytometry: Green-yellow spectrum detection channel.
- DNA/RNA Analysis: Instruments requiring accurate PCR detection across multiple channels.
Customization and Reliable Supply
We understand the critical timeframes of product development. OPTOStokes maintains an extensive selection of in-stock components and offers world-class customization for specialized instrument requirements.
- Wavelength Customization: Adjust CWL from 400nm to 850nm and FWHM from 10nm to 60nm.
- Extreme Blocking: We guarantee ODave ≥ 6 suppression from 350-800nm for absolute out-of-band rejection.
- Form Factor Flexibility: Available in standard dimensions (6x6mm, Ø6mm, Ø12mm, Ø25mm) and custom shapes, including mounting options such as aluminum holders.
Guaranteed Performance for Technical Decision-Makers
Engineers choose OPTOStokes for guaranteed quality and controlled lead times. When signal bleed-through or unreliable quality is not an option, our robust high OD bandpass filters provide a solution.
Ready to integrate world-class fluorescence filters into your system? Contact us today at sales@optofilters.com for detailed specifications, samples, or to discuss your unique customization project.
